The efficacy of 6-bromo-3-pyridyl hydrazine on the bio-production of Lactic acid


Dr. Archana Kumari

SRPS Govt. School, Gardanibagh, Patna Magadh University, Bodh Gaya



The efficacy of chemical mutagen, i.e., 6-bromo-3-pyridyl hydrazine on the lactic acid fermentation by the lactic acid bacteria such as Lactococcus lactis NCIM-1158, Lactobacillus brevis NCIM-1263, Lactobacillus pentosus NCIM-1295, Lactobacillus fructivorans NCIM-1687 has been assessed. It has been found that the lactic acid bacteria under trial, i.e., lactobacillus brevis NCIM-1263 has been found best lactic acid producer. Therefore, these bacteria has been used throughout the present investigation. The compound,i.e.,6-bromo-3-pyridyl hydrazine as its molar concentration, i.e.,6×10-5M has been found stimulatory for the bio production of lactic acid and has been found higher in comparison to control to an extent of 8.204%(7.834g/100ml) under optimized conditions of 20%(W/V) molasses, 35°C temperature, 6.0pH and incubation period of 6 days along with other nutritional ingredients required by the Lactic acid bacteria under investigation. Keywords: Lactic acid fermentation, mutagen, 6-bromo-3-pyridyl hydrazine and Lactobacillus brevis NCIM-1263.